mouse anti marco Search Results


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R&D Systems anti mouse marco apc conjugated
Anti Mouse Marco Apc Conjugated, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse marco fluorescein  (Bio-Techne corporation)
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Bio-Techne corporation anti mouse marco fluorescein
Baseline surface expression of a CD36 stained with anti-mouse CD36-BB515 antibody; b Macrophage Receptor with Collagenous structure <t>(MARCO)</t> stained with anti-mouse <t>MARCO-Fluorescein</t> antibody; and c Macrophage Scavenger Receptor 1 (MSR1), also known as CD204, stained with anti-mouse CD204-PE antibody on wildtype and Tlr4 −/− macrophages. Receptor expression was measured using flow cytometry. Data is representative of three independent experiments which gave similar results. Numbers above gates refer to the percentage of CD36-, MARCO- and MSR1-positive cells. d MPI cells, a non-transformed GM-CSF-dependent murine macrophage cell line, isolated from wildtype, Msr1 −/− , Marco −/− and MSR1/MARCO double knockout (DKO) mice, were infected with non-opsonised C. neoformans . The data shown is pooled from three independent experiments ( n = 9). Data is mean ± SEM; statistical significance was evaluated using a one-way ANOVA; P -values are shown above the graph. Source data are provided as a Source Data file.
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marco rat ed31 abd serotec mca1849  (Bio-Rad)
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Baseline surface expression of a CD36 stained with anti-mouse CD36-BB515 antibody; b Macrophage Receptor with Collagenous structure <t>(MARCO)</t> stained with anti-mouse <t>MARCO-Fluorescein</t> antibody; and c Macrophage Scavenger Receptor 1 (MSR1), also known as CD204, stained with anti-mouse CD204-PE antibody on wildtype and Tlr4 −/− macrophages. Receptor expression was measured using flow cytometry. Data is representative of three independent experiments which gave similar results. Numbers above gates refer to the percentage of CD36-, MARCO- and MSR1-positive cells. d MPI cells, a non-transformed GM-CSF-dependent murine macrophage cell line, isolated from wildtype, Msr1 −/− , Marco −/− and MSR1/MARCO double knockout (DKO) mice, were infected with non-opsonised C. neoformans . The data shown is pooled from three independent experiments ( n = 9). Data is mean ± SEM; statistical significance was evaluated using a one-way ANOVA; P -values are shown above the graph. Source data are provided as a Source Data file.
Marco Rat Ed31 Abd Serotec Mca1849, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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marco  (R&D Systems)
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R&D Systems marco
(A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for <t>exhaustion</t> <t>(CD38,</t> <t>MARCO,</t> PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.
Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti marco antibody  (R&D Systems)
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R&D Systems anti marco antibody
(A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for <t>exhaustion</t> <t>(CD38,</t> <t>MARCO,</t> PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.
Anti Marco Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti marco polyclonal goat igg biotin  (R&D Systems)
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R&D Systems anti marco polyclonal goat igg biotin
(A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for <t>exhaustion</t> <t>(CD38,</t> <t>MARCO,</t> PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.
Anti Marco Polyclonal Goat Igg Biotin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti marco  (R&D Systems)
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(A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for <t>exhaustion</t> <t>(CD38,</t> <t>MARCO,</t> PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.
Goat Anti Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse marco  (R&D Systems)
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(A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for <t>exhaustion</t> <t>(CD38,</t> <t>MARCO,</t> PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.
Goat Anti Mouse Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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marco ab  (R&D Systems)
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R&D Systems marco ab
(A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for <t>exhaustion</t> <t>(CD38,</t> <t>MARCO,</t> PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.
Marco Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse marco  (R&D Systems)
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A continuing lung resolution process at 25 dpii characterized by AMs and type II epithelial cell phenotype in the lung. Mice were infected i.n. with a dose of 20 pfu of the influenza virus PR8 (data labeled as blue) or administered with PBS (data labeled as black) and analyzed after 25 days. ( A ) (I) Representative FACS plots <t>of</t> <t>SiglecF/CD11c-positive</t> AMs extracted from whole lungs of influenza-infected mice (25 dpii) compared to those extracted from whole lungs of naïve mice, followed by a summary of alveolar macrophage percentages (II) and absolute numbers (III). n = 16–19 (II) and 6–7 (III) mice per group. ( B ) Representative flow cytometry histograms and ( C ) percentage of SiglecF/CD11b AMs expressing CD220R (I) TREM-2 (II) <t>MARCO</t> (III) in the lungs of PBS (black) and 25 dpii (blue). n = 8–16 mice per group. Immunofluorescence images of lungs naïve or influenza infected lungs (25 dpii) immune-stained for T1α ( D ) and proSPC ( E ). Mean fluorescence intensity quantification of T1 α ( F ) and proSPC ( G ) and the number of proSPC-positive cells ( H ) per field of view as quantified using Zen1 software (Zeiss). Graphs show the mean ± SEM intensity of 10–14 fields per mouse. Scale bar, 50 µm. ** p < 0.01, *** p < 0.001 vs naïve.
Anti Mouse Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Baseline surface expression of a CD36 stained with anti-mouse CD36-BB515 antibody; b Macrophage Receptor with Collagenous structure (MARCO) stained with anti-mouse MARCO-Fluorescein antibody; and c Macrophage Scavenger Receptor 1 (MSR1), also known as CD204, stained with anti-mouse CD204-PE antibody on wildtype and Tlr4 −/− macrophages. Receptor expression was measured using flow cytometry. Data is representative of three independent experiments which gave similar results. Numbers above gates refer to the percentage of CD36-, MARCO- and MSR1-positive cells. d MPI cells, a non-transformed GM-CSF-dependent murine macrophage cell line, isolated from wildtype, Msr1 −/− , Marco −/− and MSR1/MARCO double knockout (DKO) mice, were infected with non-opsonised C. neoformans . The data shown is pooled from three independent experiments ( n = 9). Data is mean ± SEM; statistical significance was evaluated using a one-way ANOVA; P -values are shown above the graph. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Toll-like receptor 4 and macrophage scavenger receptor 1 crosstalk regulates phagocytosis of a fungal pathogen

doi: 10.1038/s41467-023-40635-w

Figure Lengend Snippet: Baseline surface expression of a CD36 stained with anti-mouse CD36-BB515 antibody; b Macrophage Receptor with Collagenous structure (MARCO) stained with anti-mouse MARCO-Fluorescein antibody; and c Macrophage Scavenger Receptor 1 (MSR1), also known as CD204, stained with anti-mouse CD204-PE antibody on wildtype and Tlr4 −/− macrophages. Receptor expression was measured using flow cytometry. Data is representative of three independent experiments which gave similar results. Numbers above gates refer to the percentage of CD36-, MARCO- and MSR1-positive cells. d MPI cells, a non-transformed GM-CSF-dependent murine macrophage cell line, isolated from wildtype, Msr1 −/− , Marco −/− and MSR1/MARCO double knockout (DKO) mice, were infected with non-opsonised C. neoformans . The data shown is pooled from three independent experiments ( n = 9). Data is mean ± SEM; statistical significance was evaluated using a one-way ANOVA; P -values are shown above the graph. Source data are provided as a Source Data file.

Article Snippet: The following fluorochrome-conjugated antibodies were used: 0.5 μg/mL anti-mouse CD45-PerCP-Cyanine5.5 [ThermoFisher; Cat#: 45-0451-82; Clone 30-F11], 0.25 μg/100 μL anti-mouse CD204(MSR1)-PE [Fisher Scientific; Cat#: 12-204-682; Clone M204PA], 0.25 μg/100 μL anti-mouse CD36-BB515 [BD Biosciences; Cat#: 565933; Clone CRF D-2712], and 10 μL/100 μL anti-mouse MARCO-Fluorescein [Biotechne; Cat#: FAB2956F; Clone 579511].

Techniques: Expressing, Staining, Flow Cytometry, Transformation Assay, Isolation, Double Knockout, Infection

(A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for exhaustion (CD38, MARCO, PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.

Journal: Cell reports

Article Title: Altered DNA methylation underlies monocyte dysregulation and immune exhaustion memory in sepsis

doi: 10.1016/j.celrep.2024.113894

Figure Lengend Snippet: (A) scRNA-seq UMAP feature plots for transcription factor TCF7L2 motif enrichment and gene expression. Numbers indicate different cell clusters, as outlined in . (B) RT-qPCR for key exhaustion genes in BMMCs under PBS control or repetitive LPS stimulation in the presence of different concentrations of Wnt agonist 1. Expression levels were normalized to the geometric mean of Ube2l3 , Oaz1 , and Nktr (mean expression ± SD; n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test; ****p-adj < 0.0001; ***p-adj < 0.001; **p-adj < 0.01; *p-adj < 0.05; ns, not significant; differences are not significant unless otherwise specified; see for exact p values). (C) Flow cytometry mean fluorescence intensity (MFI) for exhaustion (CD38, MARCO, PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) and population statistics (bottom) for non-classical (Ly6C low ), intermediate (Ly6C int ), and classical (Ly6C high ) monocyte subtypes in cultured BMMCs (n = 6–13; one-way ANOVA with Sidak’s multiple comparisons test). (E) NAD + levels in cultured BMMCs normalized to total protein levels (n = 3–6; one-way ANOVA with Sidak’s multiple comparisons test). (F) Correlation heatmap for average DNA methylation levels at key exhaustion loci in cultured BMMCs (n = 3–9 for each condition). (G) UCSC browser track views of average DNA methylation at the Plac8 promoter (chr5: 100,572,230–100,572,607), Cebpa/g distal enhancer (chr7: 35,064,805–35,065,304), and Tcf7l2 intron (chr19: 55,768,986–55,769,585). ENCODE annotated promoters (red) and enhancers (orange) are depicted in the bottom track.

Article Snippet: For Panel 2, cells were stained with antibodies against Ly6C (Pacific Blue; Biolegend #128014), CD11b (BV650; BD Biosciences #563402), Ly6g (PE-Cy5.5; Elabscience #E-AB-F1108I), CXCR2 (Alexa Fluor 488; R&D Systems # FAB2164G), CD68 (APC-Fire750; Biolegend #137041), CD172a (BV510; BD Biosciences #740159), CX3CR1 (PE-Cy7; Biolegend #149016), F4/80 (BV711; BD Biosciences #565612), CD38 (BV750; BD Biosciences #747103), PD-L1 (BV421; BD Biosciences #564716), MARCO (Alexa Fluor 647; R&D Systems #FAB29561R), FAS (BV605; Biolegend #152612), MHCII (PerCP; Biolegend #107624), CD168 (PE; Novus #NBP1–76538PE), and TREM2 (Alexa Fluor 700; R&D Systems #FAB17291N).

Techniques: Expressing, Quantitative RT-PCR, Control, Flow Cytometry, Fluorescence, Cell Culture, DNA Methylation Assay

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Altered DNA methylation underlies monocyte dysregulation and immune exhaustion memory in sepsis

doi: 10.1016/j.celrep.2024.113894

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For Panel 2, cells were stained with antibodies against Ly6C (Pacific Blue; Biolegend #128014), CD11b (BV650; BD Biosciences #563402), Ly6g (PE-Cy5.5; Elabscience #E-AB-F1108I), CXCR2 (Alexa Fluor 488; R&D Systems # FAB2164G), CD68 (APC-Fire750; Biolegend #137041), CD172a (BV510; BD Biosciences #740159), CX3CR1 (PE-Cy7; Biolegend #149016), F4/80 (BV711; BD Biosciences #565612), CD38 (BV750; BD Biosciences #747103), PD-L1 (BV421; BD Biosciences #564716), MARCO (Alexa Fluor 647; R&D Systems #FAB29561R), FAS (BV605; Biolegend #152612), MHCII (PerCP; Biolegend #107624), CD168 (PE; Novus #NBP1–76538PE), and TREM2 (Alexa Fluor 700; R&D Systems #FAB17291N).

Techniques: Blocking Assay, Negative Control, Recombinant, Methylation, SYBR Green Assay, DNA Methylation Assay, Software, Isolation, Reverse Transcription, Multiplex Assay

A continuing lung resolution process at 25 dpii characterized by AMs and type II epithelial cell phenotype in the lung. Mice were infected i.n. with a dose of 20 pfu of the influenza virus PR8 (data labeled as blue) or administered with PBS (data labeled as black) and analyzed after 25 days. ( A ) (I) Representative FACS plots of SiglecF/CD11c-positive AMs extracted from whole lungs of influenza-infected mice (25 dpii) compared to those extracted from whole lungs of naïve mice, followed by a summary of alveolar macrophage percentages (II) and absolute numbers (III). n = 16–19 (II) and 6–7 (III) mice per group. ( B ) Representative flow cytometry histograms and ( C ) percentage of SiglecF/CD11b AMs expressing CD220R (I) TREM-2 (II) MARCO (III) in the lungs of PBS (black) and 25 dpii (blue). n = 8–16 mice per group. Immunofluorescence images of lungs naïve or influenza infected lungs (25 dpii) immune-stained for T1α ( D ) and proSPC ( E ). Mean fluorescence intensity quantification of T1 α ( F ) and proSPC ( G ) and the number of proSPC-positive cells ( H ) per field of view as quantified using Zen1 software (Zeiss). Graphs show the mean ± SEM intensity of 10–14 fields per mouse. Scale bar, 50 µm. ** p < 0.01, *** p < 0.001 vs naïve.

Journal: Scientific Reports

Article Title: Influenza virus infection augments susceptibility to respiratory Yersinia pestis exposure and impacts the efficacy of antiplague antibiotic treatments

doi: 10.1038/s41598-020-75840-w

Figure Lengend Snippet: A continuing lung resolution process at 25 dpii characterized by AMs and type II epithelial cell phenotype in the lung. Mice were infected i.n. with a dose of 20 pfu of the influenza virus PR8 (data labeled as blue) or administered with PBS (data labeled as black) and analyzed after 25 days. ( A ) (I) Representative FACS plots of SiglecF/CD11c-positive AMs extracted from whole lungs of influenza-infected mice (25 dpii) compared to those extracted from whole lungs of naïve mice, followed by a summary of alveolar macrophage percentages (II) and absolute numbers (III). n = 16–19 (II) and 6–7 (III) mice per group. ( B ) Representative flow cytometry histograms and ( C ) percentage of SiglecF/CD11b AMs expressing CD220R (I) TREM-2 (II) MARCO (III) in the lungs of PBS (black) and 25 dpii (blue). n = 8–16 mice per group. Immunofluorescence images of lungs naïve or influenza infected lungs (25 dpii) immune-stained for T1α ( D ) and proSPC ( E ). Mean fluorescence intensity quantification of T1 α ( F ) and proSPC ( G ) and the number of proSPC-positive cells ( H ) per field of view as quantified using Zen1 software (Zeiss). Graphs show the mean ± SEM intensity of 10–14 fields per mouse. Scale bar, 50 µm. ** p < 0.01, *** p < 0.001 vs naïve.

Article Snippet: AMs (SiglecF/CD11c) were stained with PerCP-efluor710-conjugated anti-mouse SiglecF (clone 1RNM44N) (eBioscience), APC-conjugated anti-mouse CD11c (clone N418) (eBioscience), PE-conjugated anti-mouse CD220R (clone OX110) (eBioscience), FITC-conjugated anti-mouse MARCO (cat# FAB2956F) (R&D), and PE-conjugated anti-mouse TREM-2 (cat# FAB17291P) (R&D) antibodies.

Techniques: Infection, Labeling, Flow Cytometry, Expressing, Immunofluorescence, Staining, Fluorescence, Software